Discover our cutting edge technology
in the fields of Biopharmaceuticals
MSC lysis upon injection
Technology
CelliXiO explore all mechanical cell deformations in fluidic bioprocess devices, like pumps tubes and bioreactors.
These mechanical deformations, generate Shear Stress – a Physics generic term – which intensity, induces various cellular stress responses affecting Cell Physiology.
100% patented technology
At CelliXiO , innovation is at the core of our expertise. Our fully patented technology ensures exclusive, cutting-edge solutions for analyzing and mitigating shear stress. Designed for high-impact industrial applications, our advancements set new standards in low-shear bioprocessing, offering unparalleled precision and reliability.
Shear (tube)
We utilize our proprietary cell-based shear stress sensors to deliver real-time insights into shear conditions faced by mammalian cells. This allows for timely adjustments in cultivation processes, enhancing cell viability. Our service is designed to eliminate the complexities of traditional assessment methods, making outcomes accessible and actionable.
Contraction (peristaltic pump)
By leveraging shear assessment data, we work closely with clients to identify key parameters affecting cell cultivation. Our guided approach involves optimizing mixing rates, agitation strategies, and bioreactor designs tailored to specific cell types, ensuring an efficient production process. Implementing our recommendations leads to improved outcomes and reduced costs in biomanufacturing.
Compression (cell sorter)
Our training programs cover key concepts in shear stress dynamics, applicable modeling techniques, and best practices in bioprocess management. Custom workshops and seminars can be designed to meet specific organizational needs. Participants will gain valuable insights and practical skills tailored for immediate application in their research or production environments.
Mechanical impact (cell injection)
Our consulting services connect clients with experienced bioprocess experts who provide tailored insights and solutions for their unique challenges. We guide organizations in developing robust, scalable bioprocesses that mitigate shear stress impacts. Our strategic recommendations are based on the latest research and technological advancements in cell cultivation.
The physiological effect of cell deformation depend on:
- Intensity of the Shear Stress (kPa)
- Duration of the constraint
- Cell type
- Cell History and origin
With severity
ranging from:
- Cell Lysis
- Apoptosis
- Reduced viability
- Senescence
- Differentiation (Stem cells)
Characterisation of Cell sensitivity to Shear Stress
Only one run is required for an entire multistep process using different means of fluid transfer (for example: tubes + pumps + centrifugation + Immunoselection… + final injection)
Sensitivity to Shear Stress, using Cellixio Cellular Shear stress analyzer technology:
Critical Shear Stress (CSS) of 13 different cells types.
Read out:
•Viability (FACS Canto, cells labelled with iodide propidium)
•Shear Stress Intensity in kPa.
3 different sensitivity profiles depending on the type of cell
Comparison of sensitivity to Shear stress of MSCs, cancer & cellLines, and Epithelial cells
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